polyclonal rabbit anti human spns2 antibodies  (Novus Biologicals)

Journal: Medicina

Article Title: Prognostic Significance of Cytoplasmic SPNS2 Expression in Patients with Oral Squamous Cell Carcinoma

doi: 10.3390/medicina57020164

Figure Lengend Snippet: Demographics and characteristics of patients with oral squamous cell carcinoma.

Article Snippet: After deparaffinization and hydration using ethanol at various concentrations, the TMAs were subjected to antigen retrieval using 0.01 M citrate buffer (pH 6.0) in a microwave, and incubated sequentially in 3% H 2 O 2 to inhibit endogenous peroxidase activity and then in 10% normal goat serum at 37 °C for 1 h. The TMAs were then mixed with solution containing polyclonal rabbit anti-human SPNS2 antibodies (Dilution 1:100×; Catalog number: NBP1-54345; Novus Biologicals, Littleton, CO, USA) at 4 °C overnight.

Techniques: Staining

Journal: Medicina

Article Title: Prognostic Significance of Cytoplasmic SPNS2 Expression in Patients with Oral Squamous Cell Carcinoma

doi: 10.3390/medicina57020164

Figure Lengend Snippet: Overexpression of SPNS2 protein in primary OSCC tissues as detected by immunohistochemical staining. Representative immunohistochemical staining of SPNS2 protein indicating negative or positive cytoplasmic expression: ( left ) Normal tissue from adjacent to the tumor, and ( right ) Primary tumor tissue. Magnification 100× ( top ) and 400× ( bottom ). Scale bars: 25 μm ( top ) and 100 μm ( bottom ).

Article Snippet: After deparaffinization and hydration using ethanol at various concentrations, the TMAs were subjected to antigen retrieval using 0.01 M citrate buffer (pH 6.0) in a microwave, and incubated sequentially in 3% H 2 O 2 to inhibit endogenous peroxidase activity and then in 10% normal goat serum at 37 °C for 1 h. The TMAs were then mixed with solution containing polyclonal rabbit anti-human SPNS2 antibodies (Dilution 1:100×; Catalog number: NBP1-54345; Novus Biologicals, Littleton, CO, USA) at 4 °C overnight.

Techniques: Over Expression, Immunohistochemical staining, Staining, Expressing

Journal: Medicina

Article Title: Prognostic Significance of Cytoplasmic SPNS2 Expression in Patients with Oral Squamous Cell Carcinoma

doi: 10.3390/medicina57020164

Figure Lengend Snippet: Clinicopathologic variables correlated with SPNS2 expression in patients with oral squamous cell carcinoma.

Article Snippet: After deparaffinization and hydration using ethanol at various concentrations, the TMAs were subjected to antigen retrieval using 0.01 M citrate buffer (pH 6.0) in a microwave, and incubated sequentially in 3% H 2 O 2 to inhibit endogenous peroxidase activity and then in 10% normal goat serum at 37 °C for 1 h. The TMAs were then mixed with solution containing polyclonal rabbit anti-human SPNS2 antibodies (Dilution 1:100×; Catalog number: NBP1-54345; Novus Biologicals, Littleton, CO, USA) at 4 °C overnight.

Techniques: Expressing, Staining

Journal: Medicina

Article Title: Prognostic Significance of Cytoplasmic SPNS2 Expression in Patients with Oral Squamous Cell Carcinoma

doi: 10.3390/medicina57020164

Figure Lengend Snippet: Kaplan–Meier survival analysis of negative and positive cytoplasmic SPNS2 protein expression in stage III/IV OSCC patients for use in log-rank tests of homogeneity using Kaplan–Meier curves. * p < 0.05.

Article Snippet: After deparaffinization and hydration using ethanol at various concentrations, the TMAs were subjected to antigen retrieval using 0.01 M citrate buffer (pH 6.0) in a microwave, and incubated sequentially in 3% H 2 O 2 to inhibit endogenous peroxidase activity and then in 10% normal goat serum at 37 °C for 1 h. The TMAs were then mixed with solution containing polyclonal rabbit anti-human SPNS2 antibodies (Dilution 1:100×; Catalog number: NBP1-54345; Novus Biologicals, Littleton, CO, USA) at 4 °C overnight.

Techniques: Expressing

Journal: Medicina

Article Title: Prognostic Significance of Cytoplasmic SPNS2 Expression in Patients with Oral Squamous Cell Carcinoma

doi: 10.3390/medicina57020164

Figure Lengend Snippet: Overall survival of III/IV stage and clinicopathologic variables of patients with oral squamous cell carcinoma using univariate and multivariate analysis.

Article Snippet: After deparaffinization and hydration using ethanol at various concentrations, the TMAs were subjected to antigen retrieval using 0.01 M citrate buffer (pH 6.0) in a microwave, and incubated sequentially in 3% H 2 O 2 to inhibit endogenous peroxidase activity and then in 10% normal goat serum at 37 °C for 1 h. The TMAs were then mixed with solution containing polyclonal rabbit anti-human SPNS2 antibodies (Dilution 1:100×; Catalog number: NBP1-54345; Novus Biologicals, Littleton, CO, USA) at 4 °C overnight.

Techniques: Expressing